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Epigenetic modifications refer to functional changes in DNA structural packaging or associated proteins without structural alteration of the DNA sequence itself (Jaenisch & Bird, Reference Jaenisch and Bird2003)

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Basic surface patches mediate SPOC binding to phosphorylated serine To test whether SPOC domains are universal CTD binders and determine their binding specificity, we expressed and purified the SPOC domains of the human proteins PHF3, DIDO, SHARP, RBM15, and SPOCD1 and performed fluorescence anisotropy assays to measure their binding to Atto488-labeled CTD-diheptapeptides (Fig