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Fifty-four male Sprague–Dawley rats weighing 225–300 g (Hana Laboratories) were used in this study

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Dosing and Administration Standard Dose : 50 mg once daily or as directed by a licensed healthcare provider Route : Oral capsule Timing : Preferably taken in the morning with or without food Duration : Often used in 4–8 week cycles depending on clinical goals Because 5-Amino-1MQ modulates key metabolic pathways, it is strongly recommended that patients be monitored by a practitioner, especially when used alongside other metabolic or NAD⁺ therapies