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L. fermentum ME-3 / "Shirayuki Lactic Acid Bacteria" (live bacteria) セティ | IPROS GMS

The NAD + -to-NADH and ATP-to-ADP ratios were calculated from the peak area values of each metabolite within the same individual sample and compared between groups

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Results Spectroscopic characterization of T94 2.61 I/E134 3.49 Q, T94 2.61 I/M257 6.40 Y, E134 3.49 Q/M257 6.40 Y and T94 2.61 I/E134 3.49 Q/M257 6.40 Y mutants We expressed the recombinant opsins corresponding to double and triple mutants combining individual point mutations T94 2.61 I, E134 3.49 Q and M257 6.40 Y in COS-1 cells and immunopurified with the Rho-1D4 antibody in 2 mM Na 2 HPO 4 , pH 6.0, 0.05% DDM (see “Materials and Methods” section)